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Microbiological indicators and heavy metal concentration in ecological assessment of urban soils of Saint Petersburg, Russia

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This paper aimed to characterize urbostratozems (Urbic Technosol, WRB) of Saint Petersburg located in industrial (“Electrodepo” railway station) and residential (region Polish Garden) zones. These soils were also compared with background (natural) soddy podzol soil (Umbric Albic Gleic Podzol, WRB) sampled in recreational zone (suburban park “Oranienbaum”). Soil samples were collected from soil horizons for chemical analysis and from top of soils for microbialogical analysis in June of 2012. Chemical properties (pH, total organic carbon, mobile forms of K and P) and content of heavy metals (Pb, Cu, Zn, Ni) in soils were determined. Culturable forms of microorganisms (bacteria and fungi) were studied. Assessment of the enzymatic activity of the soil was carried out by culturing of microorganisms-producers of protease, amylase, cellulase and lipase on special media. Biotesting using cress (Lepidium sativum L.) seeds had been carried out for assessment of soil phytotoxicity. It was found that chemical properties of urban and natural soils differ greatly. Heavy metal pollution was evident in both urban soils, but maximum concentrations of heavy metals were found in the soil of the industrial zone. Phytotoxocity had been also most pronouncend in the soil of the industrial zone. The natural soil exhibited significantly higher respiration activity than urbostratozems. The greatest difference in the structure of the bacterial and fungal communities was observed between the natural soil of the recreational zone and the urbostratozem of the industrial zone. Algae had been present in the urban soils of the residential zone that was not observed in the natural podzol. The minimum number of producers of all enzymes, except for cellulase, was observed in the soddy podzol in the recreational zone. The maximum number of protease and amylase producers was found in the soil of the industrial zone. Lipolytic activity was almost the same in all samples. It was found that more sensitive biological methods are needed for environmental assessment of urban soils. The results of the article can be used by soil scientists and environmental engineers for a comprehensive environmental assessment of the condition of urban soils and for creating new urban green spaces.

About the Authors

Natalia N. Matinian
Saint Petersburg University
Russian Federation
Universitetskaya nab., 7-9, Saint Petersburg, 199034

Anastasia L. Gusareva
Saint Petersburg University
Russian Federation
Universitetskaya nab., 7-9, Saint Petersburg, 199034

Kseniia A. Bakhmatova
Saint Petersburg University
Russian Federation
Universitetskaya nab., 7-9, Saint Petersburg, 199034

Anastasia A. Sheshukova
Saint Petersburg University
Russian Federation
Universitetskaya nab., 7-9, Saint Petersburg, 199034


1. Aleksandrovskiy A., Dolgikh A. and Aleksandrovskaya E. (2012). Pedogenetic features of habitation deposits in ancient towns of European Russia and their alteration under different natural conditions. Boletin de la Sociedad Geologica Mexicana, 64(1), 71-77. DOI: 10.18268/ BSGM2012v64n1a6.

2. Bityukova V., Vlasov D., Dorokhova M., Kasimov N., Kislyakova N., Kirillov P., Kosheleva N., Nikiforova E., Petukhova N., Ryzhov A., Savoskul M., Saul’skaya T. and Shartova N. (2016). East – West of Moscow: a spatial analysis of social and environmental issues. Ed. by Kasimov N. Moscow: Faculty of Geography, Lomonosov Moscow State University. Bouchez T., Blieux A., Dequiedt S., Domaizion I., Dufresne A., Ferreira S., Godon J., Hellal J., Joulian C., Quaiser A., Martin-Laurent F., Mauffret A., Monier J., Peyret P., Schmitt-Koplin P., Sibourg O., D’oiron E., Bispo A., Deportes I., Grand C., Cuny P., Maron P. and Ranjard L (2016). Molecular microbiology methods for environmental diagnosis. Environmental Chemistry Letters, 14, 423-441. DOI: 10.1007/s10311-016-0581-3.

3. Braun B., Böckelmann U., Grohmann E. and Szewzyk U. (2006). Polyphasic characterization of the bacterial community in an urban soil profile with in situ and culture-dependent methods. Applied Soil Ecology, 31, 267-279. DOI:10.1016/j.apsoil.200505.003.

4. Burghardt W., Morel J.and Zhang G.-L. (2015). Development of the soil research about urban, industrial, traffic, mining and military areas (SUITMA). Soil Science and Plant Nutrition, 61, supl. 1, 3-21. DOI: 10.1080/00380768.2015.1046136.

5. Calvarro L.M., de Santiago-Martin A., Gomez G.Q., Gonsallez-Huecas C., Quintana J.R., Vázquez A. et al. (2014). Biological activity in metal contaminated calcareous agricultural soils: the role of the organic matter composition and the particle size distribution. Environmental Science and Pollution Researche, 21(9), 6176-6187. DOI: 10.1007/s11356-014-2561-0.

6. Castaldi S., Butigliano F. andVirzo de Santo A. (2004) Suitability of soil microbial parameters as indicators of heavy metal pollution. Water, Air, and Soil Pollution, 158, 21-35. DOI: 10.1023/B:WATE.0000044824.88079.d9.

7. Charzynski P., Markiewicz M. and Switoniak M., eds. (2013). Technogenic soils atlas. Torun: Polish Society of Soil Science. Classification System of Russian Soils (2004). Moscow: Pochv. Inst. im. Dokuchaeva. (in Russian).

8. Dai J., Becquer T., Rouiller J., Reversat G., Bernhardt-Reversat F. and Lavelle P. (2004). Influence of heavy metals on C and N mineralization and microbial biomass in Zn-Pb-Cu- and Cd-contaminated soils. Applied Soil Ecology, 2005, 99-109. DOI: 10.1016/j.apsoil.2003.09.003.

9. Dorokhova M. (2007) Diatoms as indicators of soil conditions in oil production regions. Oceanological and Hydrobiological Studies (Poland), 36, suppl.1, 129-135.

10. Hagmann D.F., Goodey N. M., Mathieu C., Evans J., Aronson M.F.J., Gallagher F. and Krumins J.A. (2015). Effect of metal contamination on microbial enzymatic activity in soil. Soil Biology and Biochemistry, 91, 291-297. DOI: 10.1016/j.soilbio.2015.09.012.

11. Huot H., Joyner J., Córdoba A., Shaw R., Wilson M., Walker R., Muth T. and Cheng Z. (2017). Characterizing urban soils in New York City: profile properties and bacterial communities. Journal of Soils and Sediments, 17, 393-407. DOI: 10.1007/s11368-016-1552-9.

12. Hui N., Jumpponen A., Francini G., Kotze D.J., Liu X., Romantschul M., Srömmer R. and Setal H. (2017). Soil microbial communities are shaped by vegetation type and park age in cities under cold climate. Environmental Microbiology 19(3), 1281-1295. DOI: 10.1111/1462-2920.13660.

13. Hygienic Standard (2006). Approximate permissible concentrations of chemical substances in soil. (in Russian).

14. Greinert A. (2015). The heterogeneity of urban soils in the light of their properties. Journal of Soils and Sediments, 15, 1725-1737. DOI: 10.1007/s11368-014-1054-6.

15. IUSS Working group WRB (2006). World reference base for soil resources. World Soil Recourses Reports № 103. FAO, Rome.

16. IUSS Working Group WRB (2014). World Reference Base for Soil Resources 2014. International soil classification system for naming soils and creating legends for soil maps. World Soil Resources Reports No. 106. FAO, Rome.

17. Ivashchenko K., Ananyeva N., Vasenev V., Sushko S., Seleznyova A. and Kudeyarov V. (2019). Microbial C-availability and organic matter decomposition in urban soils of megapolis depend on functional zoning. Soil Environ. 38(1), 31-41. DOI: 10.25252/SE/19/61524.

18. Labutova N.(2008). Methods of studing of soil microorganisms. SPb.: izd. SPbSU. (in Russian).

19. Labutova N. and Bankina T. (2013). Basics of soil enzymology. SPb.: izd. SPbSU. (in Russian). Lehmann A. and Stahr K. (2007). Nature and significance of anthropogenic urban soils. Journal of Soils and Sediments, 7(4), 247-260. DOI: 10.1065/jss2007.06.235.

20. Marfenina O. (1987). Micromycetes as indicators of soil pollution by heavy metals. In: The influence of industry on environment. Мoscow: Nauka, 189-196. (in Russian).

21. Marfenina O. (2005). Anthropogenic ecology of soil fungi. Мoscow: Meditsina dlya vsekh. (in Russian).

22. Marfenina O., Lysak L., Ivanova A., Glushakova A., Kachalkin A., Nikolaeva V., Karlsen A. and Tepeeva A. (2017). Biodiversity in urban soils: threats and opportunities (on the example of cultivated microorganisms). Soils of Urban, Industrial, Traffic, Mining and Military Areas (SUITMA 9) 22-26 May 2017, Moscow. Abstracts, 109-111.

23. Matinian N.N., Reimann K., Bakhmatova K.A. and Rusakov A.V. (2007). The background concentration of heavy metals and As in arable soils of North-West of Russia (based on the materials of international geochemical atlas). Vestnik of Saint Petersburg state university, Seriya Biologia, 3, 123-134. (in Russian).

24. Microbiology laboratory manual (2005). Ed.: Netrusov A.I. Moscow: Academia publishing. 608 p. (in Russian). Muchlbachova G., Sagova-Maeckova M., Omelka M., Szakova J. and Tlustos P. (2015). The influence of soil organic carbon on interactions between microbial parameters and metal concentrations at a long-term contaminated site. Science of the Total Environment, 502, 218-223. DOI: 10.1016/j.scitoenv.2014.08079.

25. Nannipieri P., Ascher J., Ceccherini M.T., Landi L., Pietramellara G. and Renella G. (2003). Microbial diversity and soil function. European Journal of Soil Science, 54, 655-670. DOI: 10.1046/j.1365-2389.2003.00556.x.

26. Naylo A., Almeida Pereira S.I., Benidire L., El Khalil H., Castro P.M.L., Ouvrard S., Schwartz C. and Boularbah A. (2019). Trace and major element contents, microbial communities, and enzymatic activities of urban soils of Marrakech city along an anthropization gradient. Journal of Soils and Sediments, 19, 2153-2165. DOI: 10.1007/s11368-018-2221-y.

27. Papa S., Bartoli G., Pellegrino A. and Fioretto A. (2010). Microbial activities and trace element contents in an urban soil. Environment Monitoring Assessment, 165, 193-203. DOI: 10.1017/s10661-009-0938-1.

28. Piotrowska-Dlugosz A. and Charzyńsky P. (2015). The impact of the soil sealing degree on microbial biomass, enzymatic activity, and physicochemical properties in the Ecranic Technosols of Toruń (Poland). Journal of Soils and Sediments, 15, 47-59. DOI: 10.1007/s11368-014-0963-8.

29. Prokof´eva, T., Gerasimova M., Bezuglova O., Bakhmatova K., Gol’eva A., Gorbov S., Zharikova E., Matinyan N., Nakvasina E. and Sivtseva N. (2014). Inclusion of soils and soil-like bodies of urban territories into the Russian soil classification system. Eurasian Soil Science, 47(10), 959-967. DOI: 10.7868/S0032180X14100104.

30. Prokof”eva T. and Poputnikov V. (2010). Anthropogenic transformation of soils in the Pokrovskoe-Streshnevo Park (Moscow) and adjacent residential areas, Eurasian Soil Science, 43 (6), 701-711. DOI: 10.1134/S1064229310060116.

31. Rabotnova I. and Pozmogova I. (1979). Chemostat cultivation and inhibition of microbial growth. М.: izd. Nauka. (in Russian).

32. Rozanova M., Prokof”eva T., Lysak L. and Rakhleeva A. (2016). Soil organic matter in the Moscow State University botanical garden on the Vorob’evy Hills. Eurasian Soil Science, 49 (9), 1013-1025. DOI: 10.1134/S106422931609012X.

33. Schindelbeck R., van Es H., Abawi G., Wolfe D., Whitlow T., Cugino B., Idowu O. and Moebius-Clune B. (2008). Comprehensive assessment of soil quality for landscape and urban management. Landscape and Urban Planning, 88, 73-80. DOI: 10.1016/j.landurbplan.2008.08.006.

34. Stefanovicz A., Kapusta P. and Szarek-Lukaszewska G. (2010). Pine forest and grassland differently influence the response of soil microbial communities to metal contamination. Science of the Total Environment, 408, 6134-6141. DOI: 10.1016/j.scitotenv.2010.08.056.

35. Stefanovicz A.., Kapusta P., Szarek-Lukaszewska G., Groudzińska K., Niklińska M. and Vogt R.(2012). Soil fertility and plant diversity enhance microbial performance in metal-polluted soils. Science of the Total Environment, 439, 211-219. DOI: 10.1016/j.scitotenv.2012.09.030.

36. Szegi J. (1983). Methods of soil microbiology. Moscow: “Kolos” Publ. (in Russian). Terekhova V., Pukalchik M. and Yakovlev A.(2014). The triad approach to ecological assessment of urban soils. Eurasian Soil Science, 47(9), 952-958. DOI: 10.1134/S1064229314090129.

37. Vorobieva L., ed. (2006). Theory and practice chemical analysis of soils. Moscow: izd. MGU. (in Russian).

38. Yuangen Y., Campbell C., Clark L., Cameron C. and Paterson E. (2006). Microbial indicators of heavy metal contamination in urban and rural soils. Chemosphere, 63, 1942-1952. DOI: 10.1016/j.chemosphere.2005.10.009.

39. Yuangen Y., Paterson E. and Campbell C. (2001). Urban soil microbial features and their environmental significance as exemplified by Aberdeen City, UK. Chinese Journal of Geochemistry, 20(1), 34-44. DOI: 10.1007/BF03166847.

40. Zenova G., Shtina E., Dedysh al. (1995). Ecological connections of algae in biogeocoenoses. Microbiology, 64(2), 149-164. (in Russian).

41. Zhigareva T., Ratnikov A., Sviridenko D., Popova G., Petrov K., Kas’yanenko A., Chernykh N. and Kartuzova M. (2006). Investigation of behavior of Cd and Zn and their influence on soil microbocenoces. Vestnik of RUDN. Ser. Ecology and life safety, 1(13), 34-40. (in Russian).

42. Zvyagintsev D., Bab’eva I. and Zenova G. (2005). Soil biology. Moscow: izd. MGU. (in Russian).

For citation:

Matinian N.N., Gusareva A.L., Bakhmatova K.A., Sheshukova A.A. Microbiological indicators and heavy metal concentration in ecological assessment of urban soils of Saint Petersburg, Russia. GEOGRAPHY, ENVIRONMENT, SUSTAINABILITY. 2020;13(1):214-223.

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