Chlorophyll-a (Chl-a) is a type of pigment is most common and predominant in all oxygen-evolving photosynthetic organisms such as higher plants, red and green algae. The concentrations of high chlorophyll-a (Chl-a) in coastal waters tend to be lower offshore due to land through river water runoff. The Madura Strait is one of the Indonesian basins that is widely used for fisheries activity, which directly impacts and puts quite high pressure on the aquatic resources. In addition, the development of urban areas and changes of land use in the hinterland areas of East Java Province due to increasing population are also intensive. The objectives of this research were: (1) to map the distribution of chlorophyll-a, its concentration and dynamics in the Madura Strait near the Pasuruan coastal area using remote sensing for both dry and rainy seasons, (2) figure out the influence of rivers or other oceanographic factors that may occur, and (3) calculate the accuracy of the estimation compared to the field data. The Landsat 8 OLI imagery was used to determine the concentration of Chl-a and analyze its seasonal spatial distribution pattern. The results show that (1) spatial distribution of chlorophyll-a (Chl-a), its concentration and dynamics in the Madura Strait waters near the Pasuruan coastal area varies between dry and rainy months or seasons, (2) input from rivers, waves, tidal level, and eddy circulation constitute the oceanographic parameters that influence the spatial distribution pattern of chlorophyll-a (Chl-a) in the Madura Strait waters near the Pasuruan coastal area, and (3) validation of the estimated Chl-a concentrations from Landsat 8 OLI using field data has shown RMSE value of 0.49.